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Capitoli
- 00:05Titolo
- 00:58Generation and Expression of Fusion Proteins and Free Dam Proteins
- 03:12Library Preparation: Fragmentation
- 05:29Library Preparation: Size Selection
- 07:26Results: Detecting Lamin B1 Binding Regions on the Mouse Chromosome One
- 08:20Conclusion
We describe herein an assay by coupling DNA adenine methyltransferase identification (DamID) to high throughput sequencing (DamID-seq). This improved method provides a higher resolution and a wider dynamic range, and allows analyzing DamID-seq data in conjunction with other high throughput sequencing data such as ChIP-seq, RNA-seq, etc.
Tags
DamID-seqGenome-wide MappingProtein-DNA InteractionsHigh Throughput SequencingAdenine-methylated DNA FragmentsNuclear OrganizationTranscription RegulationMammalian CellsGenomic RegionsAmyloidTranscription FactorsHigh ResolutionDNA MicroarraysCross-analysisGene CloningDamID VectorLentiviral StocksLentiviral Expression SystemCotransfectionLentivirus Infection
