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Capitoli
- 00:05Titolo
- 00:39Cell Culture for Immunofluorescence Microscopy
- 01:32Preparation of Transfection Complex and Transfection of Cells
- 03:08Live Cell Imaging and Indirect Immunofluorescence
- 05:40Results: Identifying Kinesin Cargo via Fluorescence Microscopy
- 06:50Conclusion
Here, a protocol is presented to identify Kinesin-1 cargos. A motorless mutant of the Kinesin-1 heavy chain (KIF5B) aggregates in the cytoplasm and induces aggregation of its cargos. Both aggregates are detected under fluorescence microscopy. A similar strategy can be employed to identify cargos of other motor proteins.
