Rivista
/
Biologia dello sviluppo
/
ื ื™ืชื•ื— ื›ืžื•ืชื™ ืฉืœ ื‘ื™ื˜ื•ื™ ื—ืœื‘ื•ืŸ ืœื—ืงืจ ืžืคืจื˜ Lineage ืขื›ื‘ืจ preimplantation ืขื•ื‘ืจื™ื
JoVE Journal
Biologia dello sviluppo
รˆ necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
Please note that all translations are automatically generated. Click here for the English version.

ื ื™ืชื•ื— ื›ืžื•ืชื™ ืฉืœ ื‘ื™ื˜ื•ื™ ื—ืœื‘ื•ืŸ ืœื—ืงืจ ืžืคืจื˜ Lineage ืขื›ื‘ืจ preimplantation ืขื•ื‘ืจื™ื

DOI:
10.3791/53654-v

โ€ข

11:25 min

โ€ข

February 22, 2016

โ€ข

, , , ,
1Developmental Biology Program,Sloan Kettering Institute

Capitoli

  • 00:05Titolo
  • 00:41Embryo Collection
  • 03:25Confocal Imaging
  • 04:47Image Analysis and Data Pre-processing
  • 08:44Results: Lineage Specification Analysis in Mouse Preimplantation Embryos
  • 10:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

ืคืจื•ื˜ื•ืงื•ืœ ื–ื” ืžืฆื™ื’ ืฉื™ื˜ื” ืœื‘ืฆืข ืžืชื—ื™ืœ ืžืชื ื‘ื•ื“ื“ ื›ืžื•ืชื™, ื‘ื ื™ืชื•ื— ื‘ืืชืจื• ืฉืœ ื‘ื™ื˜ื•ื™ ื—ืœื‘ื•ืŸ ืœืœืžื•ื“ ืžืคืจื˜ ื”ืฉื•ืฉืœืช ื‘ืขื•ื‘ืจื™ื preimplantation ื”ืขื›ื‘ืจ. ื”ื ื”ืœื™ื ื”ื“ืจื•ืฉื™ื ืœืื™ืกื•ืฃ blastocysts, ื›ืœ ื”ืจ ื–ื™ื”ื•ื™ immunofluorescent ืฉืœ ื—ืœื‘ื•ื ื™ื, ื”ื“ืžื™ื” ืฉืœ ื“ื’ื™ืžื•ืช ืขืœ ืžื™ืงืจื•ืกืงื•ืค confocal, ื•ืคื™ืœื•ื— ื’ืจืขื™ื ื™ ื•ื ื™ืชื•ื— ืชืžื•ื ื” ืžืชื•ืืจืช.

Tags

Quantitative AnalysisProtein ExpressionLineage SpecificationMouse Preimplantation EmbryosConfocal MicroscopySingle-cell ResolutionSemi-automatedNuclear DensityCell Population HeterogeneityZona Pellucida RemovalImmunofluorescenceConfocal Imaging

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/53654?article_type=v

Loss- ื•-ืฉืœ-ืคื•ื ืงืฆื™ืช ืจื•ื•ื— ื’ื™ืฉื” ืœื—ืงืจ ืชื ืžื•ืงื“ื ืงื•ื‘ืขื™ ื’ื•ืจืœ preimplantation ืขื›ื‘ืจ ืขื•ื‘ืจ
check_url/it/53654?article_type=v

In vivo ื”ื“ืžื™ื” ืฉืœ ื‘ื™ื˜ื•ื™ ืžื”ื•ื ื“ืก ื’'ื™ืŸ ื”ืคืจื˜ ืจืฉืชื™ืช ืื‘ื•ืช ื‘ Chimeric ื“ื’ ื”ื–ื‘ืจื” ืขื•ื‘ืจื™ื ืœื—ืงืจ ื”ืฉืคืขื•ืช Cell Nonautonomous
check_url/it/53654?article_type=v

ืจื–ื•ืœื•ืฆื™ื” ืกื•ืคืจ correlative ื•ืืœืงื˜ืจื•ืŸ ืœืคืชืจื•ืŸ ื—ืœื‘ื•ืŸ ืœื•ืงืœื™ื–ืฆื™ื” ื‘ืจืฉืชื™ืช ื“ื’ ื–ื‘ืจื”
check_url/it/53654?article_type=v

ื ื’ื–ืจืช ืฉืœ ืฉื•ืจื•ืช ืชืื™ ื’ื–ืข ืžืขื•ื‘ืจื™ื ืฉืœ ืขื›ื‘ืจ
check_url/it/53654?article_type=v

ื ื™ืชื•ื— ื›ืžื•ืชื™ Immunofluorescence ื›ื•ืœื”-ื”ืจ ืฉืœ ืื•ื›ืœื•ืกื™ื•ืช ืœื‘ ื‘ ื”ืขื›ื‘ืจ ืขื•ื‘ืจื™
check_url/it/53654?article_type=v

ืขืงื™ื‘ื” ื‘ื™ื˜ื•ื™ ื’ื ื™ื ื“ืจืš ื–ื™ื”ื•ื™ ืฉืœ ฮฒ-galactosidase ืคืขื™ืœื•ืช ื‘ ืขื›ื‘ืจ ื›ืœ ื”ืขื•ื‘ืจื™ื
check_url/it/53654?article_type=v

ืœื”ืžื—ื™ืฉ ืืช ืฆื•ืžืช ื•ืืช ืฆืœื—ืช Notochordal Gastrulating ืขื•ื‘ืจื™ ื”ืขื›ื‘ืจ ื‘ืืžืฆืขื•ืช ืžื™ืงืจื•ืกืงื•ืค ืืœืงื˜ืจื•ื ื™ื ืกืจื™ืงื” ื›ืœ ื”ืจ Immunofluorescence
check_url/it/53654?article_type=v

ื™ืฉื™ืจ ืœืฉื•ืฉืœืช ื”ืชื›ื ื•ืช ืฉืœ ื”ืขื›ื‘ืจ ืœืžื‘ื•ื’ืจื™ื ืคื™ื‘ืจื•ื‘ืœืกื˜ Erythroid ืื‘ื•ืช
check_url/it/53654?article_type=v

ื‘ื™ื“ื•ื“ ื•ื›ืชืžื™ื ืฉืœ ื”ืขื•ืจ ื”ืขื›ื‘ืจ Keratinocytes ืขื‘ื•ืจ ื ื™ืชื•ื— ืžื—ื–ื•ืจ ื”ืชื ื”ืกืคืฆื™ืคื™ ืฉืœ ื—ืœื‘ื•ืŸ ื”ื‘ื™ื˜ื•ื™ ื”ืกืœื•ืœืจื™ ืขืœ ื™ื“ื™ ื”ืžื•ื ื™ ื”ืงื™ืงื•ืžื˜ืจื™ื”
check_url/it/53654?article_type=v

ื‘ื™ื˜ื•ื™ ืžื”ื™ืจ ื•ื™ืขื™ืœ ืฉืœ ื—ืœื‘ื•ื ื™ื ืžืจื•ื‘ื™ื ื‘ืขื•ื‘ืจื™ ื”ืขื•ืคื•ืช ื‘ืืžืฆืขื•ืช mRNA ืืœืงื˜ืจื•ืคื•ืจืฆื™ื”

Read Article