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Isolation und Analyse über Durchflusszytometrie von Immunzellen aus dem ischämischen Gehirn der Maus
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Capitoli
- 00:05Titolo
- 01:07Transcardial Perfusion and Brain Dissection
- 04:24Cerebral Tissue Dissociation
- 06:21Myelin/cell Debris Removal by Density Gradient Separation
- 07:34Flow Cytometry Immunostaining and Counting Bead Absolute Quantification
- 09:14Results: Representative Infiltrating Immune Cell Analysis in the Ischemic Mouse Brain
- 10:56Conclusion
Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping.
Tags
IsolationFlow Cytometric AnalysisImmune CellsIschemic Mouse BrainQuantificationPhenotypic CharacterizationMultiparametric Flow CytometryPost-ischemic InflammationLesion ProgressionRepairStroke OutcomeDensity Gradient CentrifugationViable Immune CellsImmunomodulatory Treatment StrategiesPeristaltic Perfusion Pump TubeCalcium-and Magnesium-free HBSS23-gauge NeedleDissection BoardPlastic TrayFore And Hind Paws20-gauge NeedlesAbdominal SkinLateral IncisionIntegumentAbdominal WallSharp Iris ScissorsLiver
