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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
 

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Article DOI: 10.3791/53676-v • 12:52 min • November 28th, 2015
November 28th, 2015 •
1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan

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Presented here is a straightforward method for the isolation and flow cytometric analysis of glioma-infiltrating peripheral blood mononuclear cells that yields time-dependent quantitative data on the number and activation status of immune cells entering the early brain tumor microenvironment.

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Isolation Flow Cytometric Analysis Glioma-infiltrating Peripheral Blood Mononuclear Cells NK Cells Galectin-1 Myeloid Cells Tumor Rejection ShRNA Knockdown GL26 Gliomas Brain Tumor Microenvironment Syngeneic C57BL/6J Mouse Brain Flow Cytometric Analysis
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