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El cultivo humanas pluripotentes y células madre neurales en un sistema de cultivo celular cerrado para la investigación básica y preclínica
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Capitoli
- 00:05Titolo
- 00:54Setting Gas Concentrations and Temperature
- 02:34Operation of Buffer Chambers
- 03:53Incubator Setup and Cell Production Facility Preparation
- 05:36Feeding Cell Cultures
- 06:23Results: Pluripotent Stem Cells Derived in Hypoxia with Sendai Virus
- 07:13Conclusion
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
Tags
CulturingHuman Pluripotent Stem CellsNeural Stem CellsEnclosed Cell Culture SystemBasic ResearchPreclinical ResearchStem Cell GrowthDefined Atmospheric ConditionsSterilityCell Culture ParametersMultipotent Stem CellsPluripotent Stem CellsConstant EnvironmentOxygen ConcentrationCarbon Dioxide ConcentrationGas Concentrations
