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Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
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Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

DOI:
10.3791/53704-v

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12:22 min

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October 26, 2016

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, , , , , , , ,
1Department of Chemical Engineering,Rowan University, 2Department of Biological Sciences,Rowan University, 3Department of Biomedical Engineering,Drexel University

Capitoli

  • 00:05Titolo
  • 01:08Poly(N-isopropylacrylamide)-g-chonodroitin Sulfate Synthesis
  • 02:16Calcium-alginate Crosslinked Microparticle Synthesis and Preparation of the Adhesives
  • 03:58Bioadhesive Mechanical Tensile Tests
  • 06:04Qualitative Cell Viability Using a Live/Dead Assay
  • 09:36Results: Evaluation of In Vitro Mechanical and Biological Studies
  • 11:02Conclusion

Summary

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An injectable tissue engineering scaffold composed of poly(N-isopropylacrylamide)-graft-chondroitin sulfate (PNIPAAm-g-CS)-containing alginate microparticles was prepared. The adhesive strength, swelling properties and in vitro biocompatibility are analyzed in this study. The characterization techniques developed here may be applicable to other thermogelling systems.

Tags

ThermogellingPoly(N-isopropylacrylamide)Chondroitin SulfateAlginate MicroparticlesHydrogel CompositeBioadhesiveNucleus PulposusTensile Mechanical TestsCellular ViabilityBiocompatibilityNIPAAMMCSTEMEDAmmonium PersulfateEmulsionCalcium Chloride

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