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Capitoli
- 00:05Titolo
- 00:33Plant Growth and Fixation
- 02:17Dehydration and Infiltration
- 03:25Block Preparation
- 05:10Sectioning and Slide Preparation for Polarized Microscope
- 07:11Results: Cell-type Specific Responses to Brassinosteroids in the Arabidopsis Root System
- 08:21Conclusion
Crystalline cellulose is an important constituent of the plant cell wall. However, its quantification at a cellular resolution is technically challenging. Here, we report the use of polarized light technology and root cross sections to obtain information of cell wall composition at a spatiotemporal resolution.
Tags
High ResolutionQuantificationCrystalline Cellulose AccumulationArabidopsis RootsCell Wall ModificationsAnatomical Cross Section PreparationCellular ResolutionPlant PhysiologyDevelopment FieldCellular CompositionWhole Organ GrowthSterilizing Arabidopsis SeedPlant AgarMediumGerminationGrowth ChamberRichardson’s SolutionSodium CacodylateGlutaraldehydeFixation Solution
