È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:46Transfection and Immunofluorescent Staining for Endogenous Centromere Kinetochore Protein Detection
- 03:30Results: Representative Immunoflurescence Analysis of Centromere Protein-A (CENP-A) Protein Expression
- 04:42Conclusion
Here we report protocols to detect endogenous and exogenous centromere-kinetochore proteins in human cells and quantify these protein levels at centromeres-kinetochores by indirect immunofluorescent staining through the use of fixation (paraformaldehyde, acetone, or methanol fixation).
Tags
Immunofluorescence AnalysisEndogenous ProteinsExogenous ProteinsCentromere-kinetochore ProteinsCentromere Protein AFlag-taggedCENP-A ProteinsLocalizationQuantificationHuman CellsTechniqueAnaphaseSeedingCultured CellsPBSReduced Serum MediumTransfectionA And B Mixture SolutionIncubationGlucose DMEMFBSAntibioticsCell Culture IncubatorCell Culture MediumFixationParaformaldehydeKb2 BufferKb1 Buffer
