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免疫染色磷酸化表位整装斑马鱼胚胎纤毛器官
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JoVE Journal Chimica
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
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免疫染色磷酸化表位整装斑马鱼胚胎纤毛器官

DOI:
10.3791/53747-v

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08:42 min

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February 19, 2016

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, ,
1Department of Biology,Virginia Commonwealth University, 2Center for Human Disease Modeling,Duke University Medical Center

Capitoli

  • 00:05Titolo
  • 00:33Embryo Fixation
  • 01:26Immunostaining Whole Embryos
  • 03:47Confocal Imaging and Processing
  • 06:37Results: Immunofluorescence of Phospho-epitopes in Whole Mount Zebrafish Ciliated Organs
  • 08:11Conclusion

Summary

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技术描述于免疫染色在整个斑马鱼胚胎磷酸的表位，然后进行在小至初级纤毛细胞结构双色荧光共焦定位。用于固定和成像技术可以定义位置和特定蛋白质的外观或活化​​的动力学。

Tags

ImmunostainingPhospho-epitopesCiliated OrgansWhole MountZebrafish EmbryosCalcium-dependent SignalingImmunolocalizationPhosphorylated ProteinsAcetylated TubulinAnti-phospho CaMKII AntibodyFluorescent Secondary AntibodyBiologia dello sviluppoCell Biology

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