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Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
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Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

DOI:
10.3791/53748-v

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11:49 min

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March 24, 2016

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, ,
1ARC Centre of Excellence in Plant Cell Walls, School of BioSciences,University of Melbourne

Capitoli

  • 00:05Titolo
  • 00:53Labeling of the Reducing End (RE) Sugar Residue of W-sol AXs with 2-aminobenzamide (2AB)
  • 02:11Generation of Xylo-oligosaccharides from 2 AB Labeled W-sol AXs
  • 03:34Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry (MALDI-TOF MS)
  • 04:48Electrospray Ionization Quadrupole Time-of-flight Mass Spectrometry (ESI-QTOF MS)
  • 06:20ESI-MSn
  • 07:30H1 Nuclear Magnetic Resonance (NMR) Spectroscopy
  • 08:45Results: Reducing End (RE) and Internal Region Glycosyl Sequence(s) of Heteroxylans
  • 10:34Conclusion

Summary

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Traduzione automatica

This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).

Tags

Keywords: Plant Wall HeteroxylansReducing EndInternal RegionGlycosal SequencesWater-soluble HeteroxylansEndoxylanase2AB LabelingMass SpectrometryNMRGlycomicsFine StructurePolysaccharide Sequencing

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