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Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
 

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

Article DOI: 10.3791/53753-v • 11:52 min • April 23rd, 2016
April 23rd, 2016 •
1, 1,2
1Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Arizona College of Medicine, 2Center for Applied Genetics and Genomic Medicine, University of Arizona College of Medicine

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Here we employ FISH methodology to track LINE-1 retrotransposition at the single nuclei level in chromosome spreads of HepG2 cell lines stably expressing synthetic LINE-1.

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LINE-1 Retrotransposition Single Nucleus Level Fluorescence In Situ Hybridization FISH Retrotransposition Rates Retrotransposition Patterns Agarose Gel TAE Buffer CY3 FITC SNeo Probe DNA Loading Buffer PCR Cleanup Kit UV Illuminator Size Increase Signal Intensity Absorbance
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