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Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
DOI:

11:39 min

April 29, 2016

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Capitoli

  • 00:05Titolo
  • 00:45Preparation of Needles and Dishes for Cell Transplantation
  • 02:13Injection of Donor Embryos
  • 03:35Cell Transplantation
  • 06:03Single Cell Transplantation
  • 07:19Embryo Mounting
  • 08:51Results: In Vivo Cell Migration in Zebrafish Embryos
  • 10:45Conclusion

Summary

Traduzione automatica

Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.

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