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用优化的n-甲基 d-葡乙胺保护恢复法制备急性脑切片
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
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用优化的n-甲基 d-葡乙胺保护恢复法制备急性脑切片

DOI:
10.3791/53825-v

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10:53 min

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February 26, 2018

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, , , , , , ,
1Cell Types Program,Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment,Swedish Neuroscience Institute

Capitoli

  • 00:04Titolo
  • 01:05Setup of the Slicing Station
  • 03:15Brain Dissection and Slicing
  • 06:32Optimized NMDG Protective Recovery Procedure
  • 08:05Results: Improved Neuronal Preservation and Reliability of Gigaohm Seal Formation with the Optimized NMDG Protective Recovery Method
  • 09:38Conclusion

Summary

Traduzione automatica

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该协议演示了一种优化的N-甲基 D-葡乙胺 (NMDG) 脑切片制备保护恢复方法的实现。一个单一的媒体配方是用来可靠地获得健康的脑切片从动物的任何年龄和不同的实验应用。

Tags

Keywords: Brain SlicesNMDG Protective Recovery MethodPatch Clamp ElectrophysiologyNeuronal PreservationAdult Mouse BrainNMDG HEPES ACSFBrain Slice Recovery ChamberHEPES ACSFAgarose Embedding

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