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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
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Biologia
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JoVE Journal Biologia
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

DOI:
10.3791/53827-v

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11:02 min

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May 27, 2016

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, , , , , ,
1Greenebaum Cancer Center,University of Maryland School of Medicine, 2Pharmaceutical Sciences,University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences,Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science,University of Pittsburgh, 6Magee Women’s Research Institute, 7Obstetrics, Gynecology, Perinatal Research Branch (NICHD),Wayne State University School of Medicine, 8Medicina,University of Maryland School of Medicine, 9Pathology,University of Maryland School of Medicine, 10Pharmacology,University of Maryland School of Medicine, 11Experimental Therapeutics,University of Maryland School of Medicine

Capitoli

  • 00:05Titolo
  • 01:04In Silico Prediction of Aternative First Exons of Bcrp1 Using BLAST Analysis of Mouse EST Database
  • 04:30Design of Reporter Constructs for Bcrp1 E1U, E1A, E1B, and E1C Promoters
  • 07:55Reporter Assay Methods
  • 09:03Results: Evaluation of Bcrp1 Alternative Promoter Function
  • 10:07Conclusion

Summary

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With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

Tags

Alternative Promoter UsageTranscriptional RegulationBcrp1MRNA ExpressionReporter AssaysGene RegulationBLASTESTIn-silico MethodsSequence Alignment

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