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高通量CRISPR载体的构建及DNA修饰的表征番茄毛状根的一代

JoVE Journal
Biologia

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JoVE Journal Biologia

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

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高通量CRISPR载体的构建及DNA修饰的表征番茄毛状根的一代

DOI:

10.3791/53843-v

•

12:59 min

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April 30, 2016

•

Thomas B. Jacobs, Gregory B. Martin²

¹Boyce Thompson Institute for Plant Research, ²Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science,Cornell University

Capitoli

00:05Titolo
00:36CRISPR Vector Construction
06:36Hairy Root Transformation
10:59Results: Hairy Root Cultures and Detection of DNA Modification
12:17Conclusion

Summary

Traduzione automatica

使用DNA装配，可以并行地构建在单个克隆反应多个CRISPR矢量，使得大量CRISPR的建设矢量a简单的任务。番茄毛状根是一个很好的模型系统来验证CRISPR载体和产生突变体材料。

Tags

Keywords: CRISPR Vector Construction DNA Modifications Tomato Hairy Roots Functional Genomics Cloning Procedure Guide RNA Knockout Mutants PCR Amplification High-Throughput DNA Assembly

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