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在毛囊披针形结局生活神经末端标记的光学监测<em>离体</em>鼠耳皮肤
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin
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在毛囊披针形结局生活神经末端标记的光学监测<em>离体</em>鼠耳皮肤

DOI:
10.3791/53855-v

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11:29 min

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April 05, 2016

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1School of Medical Sciences,University of Aberdeen, 2School of Biological & Biomedical Sciences,University of Durham

Capitoli

  • 00:05Titolo
  • 01:03Preparing Ears for Labeling
  • 03:13Lanceolate Terminal Labeling
  • 06:30Imaging Labeled Lanceolate Endings
  • 08:34Results: Labeled Nerve Terminal in Hair Follicle Lanceolate Endings
  • 10:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a novel preparation for imaging live lanceolate sensory terminals of palisade endings that innervate mouse ear skin hair follicles during staining and destaining with styryl pyridinium dyes.

Tags

Optical MonitoringLiving Nerve TerminalHair Follicle Lanceolate EndingsEx Vivo Mouse Ear SkinMechanosensory Nerve TerminalsSynaptic-like VesiclesCalcium DependenceSkin SeparationCartilage RemovalVital StainingDissection Technique

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