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Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device

Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device

DOI:
10.3791/53863-v

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11:30 min

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February 28, 2016

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, ,
1Department of Molecular Microbiology,John Innes Centre, Norwich Research Park, 2Department of Biology,Lund University

Capitoli

  • 00:05Titolo
  • 01:04Isolation of Fresh S. venezuelae Spores and Preparation of Spent Culture Medium
  • 03:04Preparation of the Microfluidic Device
  • 04:59Setup of the Microscope and the Time-lapse Protocol
  • 09:16Results: Fluorescence Time-lapse Imaging of the Complete Life Cycle Using a Microfluidic Device
  • 10:45Conclusion

Summary

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Streptomyces are characterized by a complex life cycle that has been experimentally challenging to study by cell biological means. Here we present a protocol to perform fluorescence time-lapse microscopy of the complete life cycle by growing Streptomyces venezuelae in a microfluidic device.

Tags

Keywords: Fluorescence Time-lapse ImagingStreptomyces VenezuelaeLife CycleMicrofluidic DeviceCell DifferentiationSporulationPolarized GrowthProtein LocalizationMulticenter MyceliumSporesSupplemented Growth MediumLiquid CulturePhase Contrast MicroscopyCentrifugationSpore SuspensionSpent MediumSyringe FilterMicrofluidic Plate

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