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Receptor Autoradiografi Protokoll for Lokalisert Visualisering av angiotensin II-reseptorer
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Neuroscienze
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JoVE Journal Neuroscienze
Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Receptor Autoradiografi Protokoll for Lokalisert Visualisering av angiotensin II-reseptorer

DOI:
10.3791/53866-v

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12:03 min

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June 07, 2016

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, , ,
1Farquhar College of Arts and Sciences,Nova Southeastern University, 2Department of Pharmaceutical Sciences, College of Pharmacy,Nova Southeastern University, 3School of Medicine,University of Colorado

Capitoli

  • 00:05Titolo
  • 01:13Receptor Autoradiography Tissue Section Preparation
  • 03:03Receptor Autoradiography
  • 05:35Film Exposure and Development
  • 06:49Histology and Densitometric Image Analysis
  • 08:44Results: Representative Receptor Autoradiography Imaging and Specific Binding Assessment
  • 10:38Conclusion

Summary

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Here we present a protocol to describe the localization of angiotensin II Type 1 receptors in the rat brain by quantitative, densitometric, in vitro receptor autoradiography using an iodine-125 labeled analog of angiotensin II.

Tags

Receptor AutoradiographyAngiotensin II ReceptorsBrain SectionsQuantificationLocalizationNeurodegenerative DiseasesCardiovascular DiseasesHormone Receptor SystemsDrugs Of AbuseCryostat SectioningMicroscope SlidesRadio LabelingNon-specific TreatmentTotal Treatment

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