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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
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Capitoli
- 00:05Titolo
- 01:10DNA-protein Cross-linking
- 02:05Cell Lysis and Chromatin Fragmentation
- 04:31Chromatin Immunoprecipitation
- 05:49DNA Elution, Cross-link Reversal and Proteinase K Digestion
- 07:33DNA Isolation Using DNA-binding Beads
- 10:05Results: ChIP Protocols Investigate the Epigenetic Regulation of Cardiac Gene Transcription
- 11:47Conclusion
A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.
