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Capitoli
- 00:05Titolo
- 00:45Subcutaneous (SQ) and Visceral (VIS) Fat Pad Isolation
- 02:35Fat Pad Collagenase Digestion
- 04:20Magnetic Cell Separation
- 06:31Results: Fat Depot-dependent Expression of Collagenolytic Matrix Metalloproteinases (MMPs)
- 07:52Conclusion
We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.
