/
/
Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Auditory Neuroscience Laboratory, Institute of Neuroscience of Castilla y León,University of Salamanca, 2Neural Systems Laboratory, Institute for Systems Research,University of Maryland, 3Medical Research Council Institute of Hearing Research, 4Department of Cell Biology and Pathology, Faculty of Medicine,University of Salamanca
Capitoli
- 00:05Titolo
- 00:54Tungsten Electrodes
- 03:03Multibarrel Glass Pipette Manufacturing
- 04:26Surgery and Headpost Implantation
- 06:49Electrophysiological Recording and Microiontophoresis
- 08:26Results: Effect of the Blockade of the GABAA-receptors on the Spectral and Temporal Response Properties as well as the Repetitive and Rare Sounds
- 09:58Conclusion
We present methods for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. This technique allows the detailed analysis of putative local synaptic inputs to the neuron of interest.
