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Segmentação de biofilme Associated<em> Staphylococcus aureus</em> Usando resazurina Baseado Ensaio Drogas susceptibilidade
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay
DOI:

10:00 min

May 05, 2016

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Capitoli

  • 00:05Titolo
  • 00:44Biofilm Initiation
  • 02:42Preparation of the Biofilm Challenge Plate
  • 04:20Biofilm Challenge
  • 06:03Biofilm Determination
  • 08:20Results: Viability of Biofilm-associated Cells After Antibiotic Challenge
  • 09:39Conclusion

Summary

Traduzione automatica

Most bacterial infections produce a biofilm. By virtue of their environment, biofilm associated bacteria are often phenotypically drug resistant. Novel antibacterial molecules that kill bacteria in biofilms are thus a high priority. We establish an assay to quickly screen for antimicrobial compounds that are effective at eradicating biofilms.

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