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Murine Hind Gliedmaßen langen Knochen Dissection und Knochenmark-Isolation
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
DOI:

07:17 min

April 14, 2016

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Capitoli

  • 00:05Titolo
  • 00:41Hind Limb Long Bone Dissection
  • 03:01Long Bone Preparation For Bone Marrow Isolation
  • 04:25Bone Marrow Isolation
  • 05:29Results: Representative Downstream Analyses and Applications of Rapid DIssected Mouse Long Bones and Bone Marrow
  • 06:28Conclusion

Summary

Traduzione automatica

Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

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