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1Center for Cancer Pharmacology,University of Pennsylvania, 2Center for Excellence in Environmental Toxicology,University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics,University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery,University of Pennsylvania, 5A.J. Drexel Autism Institute,Drexel University
Capitoli
- 00:05Titolo
- 00:38Preparation of Buffers and 100X Stock Solutions
- 01:35Platelet Isolation and Performing an Experiment
- 02:49Quenching and CoA Extraction
- 04:30Results: Analysis of Human Platelets to Study Mitochondrial Metabolism
- 05:21Conclusion
Här visar vi isolerade humana trombocyter kan användas som en tillgänglig ex vivo modell för att studera metaboliska anpassningar som svar på det komplexa I-inhibitor rotenon. Detta tillvägagångssätt utnyttjar isotopisk spårning och relativ kvantifiering genom vätskekromatografi-masspektrometri och kan appliceras på en mängd olika studiedesign.
