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Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation
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JoVE Journal Immunologia e infezioni
Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation

Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation

DOI:
10.3791/53964-v

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09:56 min

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March 02, 2016

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, ,
1Baylor Institute for Immunology Research,Baylor Research Institute

Capitoli

  • 00:05Titolo
  • 00:52Culturing Non-typeable Haemophilus influenzae (NTHi) and Preparing the Inoculum
  • 02:14Intra-tracheal (i.t.) Instillation
  • 03:27BALF (Broncho-alveolar Lavage Fluid) Collection
  • 05:17Homogenization of Lung Tissue and RNA Isolation
  • 07:37Results: BALF Analysis on NTHi Inoculated Mice
  • 08:47Conclusion

Summary

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We demonstrate the procedure for intra-tracheal inoculation of Haemophilus influenzae into the lower respiratory tracts of mice. This is a very useful tool to study signaling pathways that regulate airway inflammation in mouse models.

Tags

Keywords: Intra-tracheal AdministrationHaemophilus InfluenzaeMouse ModelAirway InflammationLung InflammationImmune ResponseNTHiBacterial InfectionTracheaIntubationLungEuthanasia

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