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שילוב קרינה זוגית<em> באתרו</em> כלאה עם Immunolabelling עבור איתור של הביטוי של שלושה גנים בסעיפי מוח עכבר
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
DOI:

09:23 min

March 26, 2016

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Capitoli

  • 00:05Titolo
  • 00:42Overview of Double Fluoresence In Situ Hybridization Followed by Immunostaining
  • 01:44RNA Probe Synthesis
  • 04:12Cryosectioning and Hybridization
  • 05:33Visualization of the FITC Probe and the DIG Probe
  • 07:23Immunohistochemistry and Mounting
  • 08:16Results: Expression of Aspa in Oligodendrocytes in the Cortex and the Corpus Callosum
  • 08:45Conclusion

Summary

Traduzione automatica

Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.

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