Rivista
/
/
חיזוי ותיקוף של גן בקרה אלמנטים הופעל במהלך החומצה הרטינואית מושרה גזע עובריים התמיינות תאים
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
DOI:

09:07 min

June 21, 2016

, , ,

Capitoli

  • 00:05Titolo
  • 00:45Enhancer Selection Based on ChIP-seq Analysis
  • 01:27Transfection of Embryonic Stem Cells
  • 03:21β-Galactosidase and Luciferase Assays
  • 05:19Characterization of Enhancer RNA and Measurement of eRNA Transcription
  • 06:34Results: Retinoic Acid Induced Enhancer Activation in Embryonic Stem Cells
  • 08:13Conclusion

Summary

Traduzione automatica

In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.

Video correlati

Read Article