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Samtidig Vurdering af cardiomyocyte DNA Synthesis og Ploidi: En metode til at hjælpe Kvantificering af cardiomyocyte Regeneration og Omsætning
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Samtidig Vurdering af cardiomyocyte DNA Synthesis og Ploidi: En metode til at hjælpe Kvantificering af cardiomyocyte Regeneration og Omsætning

DOI:
10.3791/53979-v

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08:03 min

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May 23, 2016

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1Institute of Genetic Medicine, International Centre for Life,Newcastle University

Capitoli

  • 00:05Titolo
  • 00:48Heart Harvest
  • 02:13Nuclei Dissociation and Cardiomyocyte Nuclei Labeling
  • 04:51EdU Incorporation Detection
  • 06:05Results: EdU Labeled Cardiomyocyte Quantification in WT and Mdx Hearts
  • 07:03Conclusion

Summary

Traduzione automatica

Traduzione automatica

Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.

Tags

CardiomyocyteDNA SynthesisPloidyRegenerationTurnoverFlow CytometryNucleoside LabelingEdUPolyploidizationCell LysisHomogenizationSucrose Gradient

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