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הערכה סימולטני של DNA Cardiomyocyte סינתזה ploidy: שיטה Assist כימות Cardiomyocyte רגנרציה מחזור
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
DOI:

08:03 min

May 23, 2016

Capitoli

  • 00:05Titolo
  • 00:48Heart Harvest
  • 02:13Nuclei Dissociation and Cardiomyocyte Nuclei Labeling
  • 04:51EdU Incorporation Detection
  • 06:05Results: EdU Labeled Cardiomyocyte Quantification in WT and Mdx Hearts
  • 07:03Conclusion

Summary

Traduzione automatica

Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.

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