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Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses
JoVE Journal
Biologia
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JoVE Journal Biologia
Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses
DOI:

07:56 min

May 03, 2016

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Capitoli

  • 00:05Titolo
  • 01:00Lens Dissection
  • 02:24Stiffness Measurements
  • 04:51Lens Nucleus Measurement and Image Analysis
  • 05:51Results: Axial and Equatorial Strain-load Curves and Morphological Characteristics of 2,4, and 8-month-old WT Mouse Lenses
  • 07:05Conclusion

Summary

Traduzione automatica

Age-related increases in eye lens stiffness are linked to presbyopia. This protocol describes a simple, cost-effective method for measuring mouse lens stiffness. Mouse lenses, like human lenses, become stiffer with age. This method is precise and can be adapted for lenses from larger animals.

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