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Bioengineering

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JoVE Journal Bioengineering
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
 

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Article DOI: 10.3791/53989-v • 10:24 min • May 9th, 2016
May 9th, 2016 •
1,2, 1, 1, 1, 2,3,4, 1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 3Department of Biomedical Engineering, University of Wisconsin-Madison, 4Morgridge Institute for Research, University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

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Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.

Tags

3D Collagen Gels Microchannels Cell Migration Cellular Processes 3D Environment Collagen Matrices Extracellular Matrix Cell Recognition Collagen Solution Neutralization Buffer Collagen Channels 3D Environment Manipulation Cell Imaging Cost-effective Equipment Collagen Mixing HEPES Buffer Cell Attachment Non-tissue Culture Surface
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