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Capitoli
- 00:05Titolo
- 01:48Preparing the CaspaseTracker Flies and Dissected Tissues
- 04:30Tissue Staining and Mounting
- 08:11Confocal Microscopy
- 09:37Results: Caspase Biosensor Activity in Non-dying Cells of Healthy Flies
- 12:10Conclusion
To detect healthy cells in whole animals that contain low levels of caspase activity, the highly sensitive biosensor designated CaspaseTracker was generated for Drosophila. Caspase-dependent biosensor activity is detected in long-lived healthy cells throughout the internal organs of adult animals reared under optimized conditions in the absence of death stimuli.
