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In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
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JoVE Journal Biologia dello sviluppo
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

DOI:
10.3791/54016-v

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09:31 min

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June 10, 2016

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, , , , , , , ,
1Diabetes and Metabolism Research Institute,Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering,California Institute of Technology

Capitoli

  • 00:05Titolo
  • 01:22Adult Murine Pancreata Single-cell Suspension Generation
  • 04:33Colony Whole Mount Immunostaining
  • 07:39Results: Representative Analyses of Colonies Derived from PCFU (Pancreatic Colony Forming Unit)
  • 08:39Conclusion

Summary

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In vitro colony assays to detect self-renewal and differentiation of progenitor cells isolated from adult murine pancreas are devised. In these assays, pancreatic progenitors give rise to cell colonies in 3-dimensional space in methylcellulose-containing semi-solid medium. Protocols for handling single cells and characterization of individual colonies are described.

Tags

Keywords: In Vitro Colony AssayTri-potent Progenitor CellsAdult Murine PancreasPancreatic Progenitor CellsSelf-renewalDifferentiationHematopoietic Stem CellsPancreatic Cell PopulationsAbdominal CavitySpleenPBSBSADNase ICollagenase BCell IsolationCell Culture

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