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用脱细胞支架龙ESC衍生鼠气道上皮细胞的生成
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Bioingegneria
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JoVE Journal Bioingegneria
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
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用脱细胞支架龙ESC衍生鼠气道上皮细胞的生成

DOI:
10.3791/54019-v

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12:31 min

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May 05, 2016

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, , , ,
1Department of Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning,Hospital for Sick Children

Capitoli

  • 00:05Titolo
  • 00:47Scaffold Preparation
  • 03:57Thick Section Generation
  • 07:17Decontamination of Scaffold Sections and Recellularization Setup
  • 09:46Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
  • 11:52Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议有效地引导小鼠胚胎干细胞衍生定形内胚层成熟呼吸道上皮细胞。这种分化技术使用的3维脱细胞肺支架直接肺谱系说明书中，在一个确定的，无血清培养的设置。

Tags

Decellularized Lung ScaffoldsEmbryonic Stem Cell DifferentiationAirway Epithelial Cells3D CultureLung DevelopmentCell Matrix ContractionsGrowth Factor SignalingSerum-free SettingRobust Airway Epithelial DifferentiationLimited Endoderm Lineage ContaminationRat LungsPerfusionTracheal CannulationDecellularization Solution

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