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Generatie van ESC afgeleide Mouse epitheelcellen behulp van cellen ontdane Lung Steigers
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Capitoli
- 00:05Titolo
- 00:47Scaffold Preparation
- 03:57Thick Section Generation
- 07:17Decontamination of Scaffold Sections and Recellularization Setup
- 09:46Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
- 11:52Conclusion
Dit protocol richt efficiënt muis embryonale stamcellen afgeleide definitieve endoderm aan de luchtwegen rijpen epitheelcellen. Deze differentiatie techniek maakt gebruik van 3-dimensionale cellen ontdane long scaffolds rechtstreekse long lijn specificatie, in een gedefinieerd serumvrij cultuurmedium setting.
Tags
Decellularized Lung ScaffoldsEmbryonic Stem Cell DifferentiationAirway Epithelial Cells3D CultureLung DevelopmentCell Matrix ContractionsGrowth Factor SignalingSerum-free SettingRobust Airway Epithelial DifferentiationLimited Endoderm Lineage ContaminationRat LungsPerfusionTracheal CannulationDecellularization Solution
