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Whole-cell Patch-clamp Recordings in Brain Slices
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JoVE Journal Neuroscienze
Whole-cell Patch-clamp Recordings in Brain Slices

Whole-cell Patch-clamp Recordings in Brain Slices

DOI:
10.3791/54024-v

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07:23 min

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June 15, 2016

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, ,
1Department of Psychiatry,University of Texas Southwestern Medical Center

Capitoli

  • 00:05Titolo
  • 00:59Slice Placement and Visualization
  • 02:15Micropipette Placement and Positioning
  • 03:45Final Approach, Seal Formation, and Obtaining the Whole-cell Configuration
  • 05:56Results: Effect of Temperature, Series Resistance, and Input Resistance on the Waveform of the Electrical Signal
  • 06:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes basic procedural steps for performing whole-cell patch-clamp recordings. This technique allows the study of the electrical behavior of neurons, and when performed in brain slices, allows the assessment of various neuronal functions from neurons that are still integrated in relatively well preserved brain circuits.

Tags

Keywords: Whole-cell Patch-clampBrain SlicesNeuroscienzeNeuron FunctionIn VitroIn VivoRecording ChamberACSFMicropipetteInternal SolutionElectrode HolderMicromanipulator

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