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Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis

Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis

DOI:
10.3791/54032-v

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07:59 min

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May 18, 2016

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,
1Department of Infection and Immunity,London School of Hygiene & Tropical Diseases

Capitoli

  • 00:05Titolo
  • 00:58Experimental Mouse Infection
  • 02:12Bioluminescence Imaging to Track Infection
  • 03:37CNS Infection Confirmation and Bioluminescence Quantitation
  • 05:02Results: Representative Bioluminescence Imaging of Infected Mice
  • 06:56Conclusion

Summary

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This manuscript describes the use of a bioluminescent strain of African trypanosomes to enable the tracking of late stage infection and demonstrates how in vivo live imaging can be used to visualize infections within the central nervous system in real-time.

Tags

Bioluminescence ImagingAfrican TrypanosomiasisSleeping SicknessTrypanosome InfectionIn Vivo Live ImagingReal-time VisualizationTherapeutic EvaluationInfectious Disease ModelsTuberculosisChagas DiseaseCD1 Female MiceTrypanosome Brucei GVR35VSL2Blood-film MicroscopyD-luciferinBioluminescent ImagingAnesthetized Animals

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