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Immunology and Infection

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JoVE Journal Immunology and Infection
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
 

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Article DOI: 10.3791/54033-v • 09:09 min • May 18th, 2016
May 18th, 2016 •
1, 2, 1, 2,3,4, 1
1Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Tech, 2Department of Biomedical Engineering, Cornell University, 3School of Electrical and Computer Engineering, Cornell University, 4Department of Biomedical Engineering, Duke University

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Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.

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Ex Vivo Culture Pattern Recognition Receptor Stimulation Mouse Intestinal Organoids Pathogenic Challenge Small Intestine Organoids Normalization Techniques Total Cell Number Innate Immunology Mucosal Immunology Host-pathogen Interactions Bacteria Intestinal Epithelial Cells In Vitro ELISA Mouse Small Intestinal Crypts Organoid Culture Glass Slide Villi Dissecting Scissors Centimeter Length Strips 50 Milliliter Conical Tube Ice Cold PBS Gentle Inversion Settle To The Bottom Of The Tube Aspirate The PBS Wash The Strips Ice Bucket Rocking Platform
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