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Udvidet Time-lapse Intravital Imaging af Real-time flercellede Dynamics i Tumor mikromiljø
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JoVE Journal Medicina
Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

Udvidet Time-lapse Intravital Imaging af Real-time flercellede Dynamics i Tumor mikromiljø

DOI:
10.3791/54042-v

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08:24 min

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June 12, 2016

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, , ,
1Department of Anatomy & Structural Biology,Albert Einstein College of Medicine, 2Department of Radiology,Albert Einstein College of Medicine, 3Gruss-Lipper Biophotonics Center,Albert Einstein College of Medicine, 4Integrated Imaging Program,Albert Einstein College of Medicine

Capitoli

  • 00:05Titolo
  • 00:40Imaging Preparation
  • 02:19Indwelling Tail Vein Catheter Insertion and Mammary Tumor Exposure
  • 03:41Image Acquisition
  • 06:03Results: Visualizing Vascular Permeability
  • 07:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol beskriver anvendelsen af multifoton mikroskopi til at udføre længere tid-lapse billeddannelse af multicellulære interaktioner i realtid, in vivo ved enkelt celle opløsning.

Tags

Intravital ImagingTumor MicroenvironmentMultiphoton MicroscopyVascular PermeabilityMulticellular InteractionsKineticsAnesthesiaTail Vein CatheterizationSubcutaneous Incision

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