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Chapters
- 00:05Title
- 00:49Preparation
- 01:58Isolation of Left Ventricular Myocytes
- 04:22Simultaneous Recordings of Ca2+ Transients and Contraction
- 06:33Analysis of the Myofilament Ca2+ Sensitivity
- 07:17Conclusion
This paper describes a protocol that assesses the changes of myofilament Ca2+ sensitivity during contraction in isolated cardiac myocytes from rat heart. Together with cardiac electrophysiology, systolic/diastolic cytosol Ca2+ levels and contraction/relaxation, this measurement is imperative in underpinning the mechanisms mediating cardiac excitation-contraction coupling in healthy and diseased hearts.
