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Π˜Π·ΡƒΡ‡Π΅Π½ΠΈΠ΅ надмолСкулярной ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΈ фотосинтСтичСских ΠΌΠ΅ΠΌΠ±Ρ€Π°Π½Π°Ρ… Π²Π½ΡƒΡ‚Ρ€ΠΈ Π€Ρ€ΠΈΠ·-Ρ‚Ρ€Π΅Ρ‰ΠΈΠ½ΠΎΠ²Π°Ρ‚Ρ‹Ρ… тканях листа с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ ΠΊΡ€ΠΈΠΎ-ΡΠΊΠ°Π½ΠΈΡ€ΡƒΡŽΡ‰Π΅ΠΉ элСктронной микроскопии
JoVE Journal
Biologia
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JoVE Journal Biologia
Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy
DOI:

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13:52 min

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June 23, 2016

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Capitoli

  • 00:05Titolo
  • 01:12Cryo-fixation of Leaf Tissues by High-pressure Freezing
  • 03:43Freeze Fracture and Double-layer Coating
  • 07:10Cryo-scanning Electron Microscopy
  • 10:52Results: Cryo-scanning Electron Microscopy Analysis of Photosynthetic Membranes in Freeze-fractured Leaf Tissues
  • 12:57Conclusion

Summary

Traduzione automatica

Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.

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