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Modellering myotonika Dystrophy 1 i C2C12 myoblastceller
 
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Modellering myotonika Dystrophy 1 i C2C12 myoblastceller

Article DOI: 10.3791/54078-v • 09:39 min • July 29th, 2016
July 29th, 2016 •
*1, *1,3, 1, 1,3, 1,2, 1
1Department of Biology and Biochemistry, University of Houston, 2Department of Economics, University of Houston, 3Department of Cardiology, The First Affiliated Hospital of Nanchang University
* These authors contributed equally

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I detta protokoll presenterar vi procedurerna i upprättandet Dystrofia myotonika 1 myoblast modeller, inklusive optimerade C2C12 cell underhåll, gen transfektion / transduktion, och myocyt differentiering.

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Medicin Dystrofia myotonika C2C12 myoblast Celf1 plasmid transfektion retroviral transduktion lentivirala transduktion immunfärgning
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