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En hurtig strategi for Isolering af nye Faustoviruses fra miljøprøver Brug<em> Vermamoeba vermiformis</em
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
DOI:

09:27 min

June 04, 2016

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Capitoli

  • 00:05Titolo
  • 00:52Host Preparation for Blind Culture Procedures and Sample Inoculation
  • 02:59Detection of Lysis by Automated Flow Cytometry
  • 04:51Preliminary Staining to Reveal the Presence of Giant Viruses and Electron Microscopy
  • 06:59Results: Detection of Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
  • 07:55Conclusion

Summary

Traduzione automatica

We describe here the latest advances in viral isolation for the characterization of new genotypes of Faustovirus, a new asfarvirus-related lineage of giant viruses. This protocol can be applied to the high throughput isolation of viruses, especially giant viruses infecting amoeba.

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