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高通量单细胞的分离和文化的微流体平台
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JoVE Journal Bioingegneria
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
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高通量单细胞的分离和文化的微流体平台

DOI:
10.3791/54105-v

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09:51 min

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June 16, 2016

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, ,
1Institute of Biomedical Engineering and Nanomedicine,National Health Research Institutes, Taiwan, 2Tissue Engineering and Regenerative Medicine,National Chung Hsing University, 3Institute of NanoEngineering and MicroSystems,National Tsing Hua University

Capitoli

  • 00:05Titolo
  • 01:35PDMS Device Preparation for Single-cell Isolation
  • 04:48Single-cell Isolation and Clonal Culture
  • 06:54Replacing the Culture Medium
  • 08:06Results: High-throughput Single-cell Isolation
  • 09:00Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

Tags

Keywords: Microfluidic PlatformSingle-cell IsolationSingle-cell CultureHigh-throughputCell HeterogeneityCell DiagnosisCancerMicrowell ArrayPDMSPhotolithographyMaster MoldCell AnalysisCell Behavior

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