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Påvisning af Anastasis In Vivo af CaspaseTracker Biosensor
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JoVE Journal Medicina
Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Påvisning af Anastasis In Vivo af CaspaseTracker Biosensor

DOI:
10.3791/54107-v

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20:16 min

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February 01, 2018

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, ,
1Institute for Basic Biomedical Sciences,Johns Hopkins University School of Medicine, 2School of Life Sciences,Chinese University of Hong Kong, 3Department of Neurosurgery,Johns Hopkins University School of Medicine

Capitoli

  • 00:05Titolo
  • 04:50Preparing the Caspase Tracker Biosensor Flies
  • 09:29Transient Cell Death Induction
  • 11:10Fixation and Staining of Egg Chambers
  • 13:58Results: Reversal of Cell Death Process in Drosophila
  • 18:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

Anastasis teknisk udfordrende for at opdage i vivo , fordi de celler, der har vendt den celle død proces kan være morfologisk umulig at skelne fra normale sunde celler. Her beskriver vi protokoller til påvisning og spore celler, der undergår anastasis i levende dyr ved hjælp af vores nyudviklede i vivo CaspaseTracker biosensor system.

Tags

AnastasisCaspaseApoptosisCell DeathCaspaseTracker BiosensorProgrammed Cell DeathRtTACre LoxPDsRedLive Cell Microscopy

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