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En Fænotype Regimen for genetisk modificerede mus bruges til at studere Gener impliceret i Human Diseases of Aging
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JoVE Journal Medicina
A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

En Fænotype Regimen for genetisk modificerede mus bruges til at studere Gener impliceret i Human Diseases of Aging

DOI:
10.3791/54136-v

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09:37 min

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July 14, 2016

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, , , , ,
1Department of Environmental Health Sciences,Yale University School of Medicine, 2Department of Ophthalmology,Yale University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:47Neonatal Hind Limb Test
  • 02:31Weanling Observation Test
  • 04:26Wire Mesh Grip Strength Test
  • 06:15Tissue Staining: NADH Diaphorase or Succinate Dehydrogenase
  • 07:47Results: HTRA2-/- Mice Testing and Histology
  • 09:08Conclusion

Summary

Traduzione automatica

Traduzione automatica

A reverse-genetics approach to understanding gene families associated with human disease is presented, using mouse as a model system, and the subsequent mouse phenotyping schedule is described. Because mice defective in a gene of interest, HtrA2, manifested Parkinsonian symptoms, the phenotyping regimen is focused on identifying neurological defects.

Tags

PhenotypingGenetically Modified MiceAgingHind Limb TestWeanling Observation TestProximal Hind Limb Muscle StrengthMuscle WeaknessMuscle FatigueBehavioral PhenotypesCellular PhenotypesHuman Disease ModelsMouse ModelsAcclimationWeight RecordingVideo RecordingStandardized Testing Setup

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