表型和慢性淋巴细胞性脉络丛脑膜炎病毒感染小鼠中分离激活调节性T细胞的功能分析

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

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表型和慢性淋巴细胞性脉络丛脑膜炎病毒感染小鼠中分离激活调节性T细胞的功能分析

DOI:

10.3791/54138-v

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07:17 min

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June 22, 2016

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Hyo Jin Park, Ji Hoon Oh, Sang-Jun Ha

¹Department of Biochemistry, College of Life Science & Biotechnology,Yonsei University

Capitoli

00:05Titolo
00:48Conventional (T_conv) and Regulatory (T_reg) Splenic T Cell Phenotyping
01:58In Vitro Suppression Assay Using CD4⁺CD25⁺ T_reg and CD8⁺ T Cells
03:16CD8⁺ T Cytokine Production and Cell Proliferation Analyses
04:26Results: Activated T_reg Cell-mediated Suppression of the CD8⁺ Responder T Cell Response
06:16Conclusion

Summary

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Here, we describe a protocol to analyze the phenotype of regulatory T (T_reg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the T_reg cells.

表型和慢性淋巴细胞性脉络丛脑膜炎病毒感染小鼠中分离激活调节性T细胞的功能分析

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