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Фенотипическая и функционального анализа активированных регуляторных Т-клеток, выделенных из хронического лимфоцитарного хореоменингита инфицированных вирусом мышей
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
DOI:

07:17 min

June 22, 2016

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Capitoli

  • 00:05Titolo
  • 00:48Conventional (Tconv) and Regulatory (Treg) Splenic T Cell Phenotyping
  • 01:58In Vitro Suppression Assay Using CD4+CD25+ Treg and CD8+ T Cells
  • 03:16CD8+ T Cytokine Production and Cell Proliferation Analyses
  • 04:26Results: Activated Treg Cell-mediated Suppression of the CD8+ Responder T Cell Response
  • 06:16Conclusion

Summary

Traduzione automatica

Here, we describe a protocol to analyze the phenotype of regulatory T (Treg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the Treg cells.

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