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Journal / Developmental Biology / Isolation of Murine Embryonic Hemogenic…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolation of Murine Embryonic Hemogenic Endothelial Cells
 

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

Capitoli

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Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.

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Isolation Murine Embryonic Hemogenic Endothelial Cells Fluorescence Activated Cell Sorting Technique Reliable Specific Viable Analysis Culture Hematopoietic Stem Cells Progenitor Cells Regulation Development Function Side Population Embryonic Tissue Dissection Scanning Procedures Work Surfaces Ethanol Euthanized Stem Supine
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